Microscopy Core

Overview

The Microscopy Core was launched in 2023 to provide training, consultation, and advanced support for optical microscopy and image analysis. Expertise and assistance are available for all steps from study design to preparation of figures and manuscripts. Instrument training and user support for scanning electron microscopy (SEM), atomic force microscopy (AFM), and laser capture microdissection (LCM) is also available.

Instruments

Widefield Microscopy:

• Nikon Eclipse Ti2-E motorized inverted microscope with white light LED illumination (brightfield and fluorescence)
• Nikon Eclipse Ti2-E motorized inverted microscope with multiline LED illumination (brightfield and fluorescence)
• Zeiss Axioscope manual upright microscope (brightfield and fluorescence)
  
  

Confocal Microscopy:

• Nikon AX R point scanning confocal
• Zeiss LSM 800 point scanning confocal
• Coming soon: Crest X-Light V3 spinning disk confocal
  
  

Specialty Microscopy:

• Asylum MFP-3D-BIO atomic force microscope (AFM)
• Hitachi S-4800 scanning electron microscope (SEM)
  
  

Image Processing and Analysis Workstations:

• NIS-Elements workstation A (standalone batch deconvolution, GA3, AI, EDF)
• NIS-Elements workstation B (integrated deconvolution, tracking, GA3, AI, EDF)
• ZEN workstation PC (Airyscan processing, BioApps)
• AFM analysis workstation PC
  
  

Services

Complimentary user support services:

• Instrument training
• Consultation in study design or imaging and analysis approaches
• Grant application and manuscript support (writing methods, building figures, etc.)
• Troubleshooting and technical assistance

Services with hourly rates:

• Image acquisition or analysis performed by core staff
• Development of automated image acquisition routines (NIS-Elements JOBS)
• Development of automated image processing or analysis tools and algorithms (NIS-Elements GA3, NIS.ai)
• Assistance during use of microscopes or workstations
  
  

Acknowledgements and Authorship

In all instances where core resources were used to acquire, process, or analyze data related to presentations and publications, the authors should acknowledge the Microscopy Core. For publications and posters, at minimum, the following statement (or similar) should be included:
“The authors acknowledge the Microscopy Core at Nationwide Children’s Hospital for providing microscopy resources and support.”
Additional details, including specific experiments, processes, or staff names, may be included as appropriate.

In instances of substantial intellectual contributions by core personnel (e.g. major role in study design/optimization, leading the development of image analysis methods and data processing, major manuscript contributions, etc.), these contributions should be reflected with authorship. In these cases, the statement of acknowledgment should also be included.

 

Leadership

Tatyana Vetter | Director

Joel Pantuso-Iwaskewycz | Microscopy Assistant

Location and hours of operation

Hours	Locations
Staffed Mon-Fri 9am-6pm  Always open to trained users	700 Children's Drive      Columbus, OH 43205      575 Children's Crossroad      Columbus, OH 43215

Links and Resources

1. Microscopy Core - Home (sharepoint.com)
2. Customer e-Learning | Nikon Instruments Inc.
3. Nikon’s MicroscopyU - The Source for Microscopy Education
4. ZEN Knowledge Base

Contacts

Name	Role	Phone	Email	Location
Microscopy Core			MicroscopyCore@nationwidechildrens.org
Tatyana Vetter	Director		Tatyana.Vetter@nationwidechildrens.org	RB4.3.419
Joel Pantuso-Iwaskewycz	Microscopy Assistant		Joel.PantusoIwaskewycz@nationwidechildrens.org	RB4.3.408C3

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