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About

Flow Cytometry Core


Flow Cytometry

The flow cytometry core lab located in W290 at The Research Institute at Nationwide Children’s Hospital offers several platforms on which to do flow analysis and/or cell sorting.  Depending on the complexity of the experiment involved there are several “levels” of cytometer available to address your specific needs.  In addition, Flow Jo analysis software is available on stand alone computers on both Macintosh and PC platforms.  Flow cytometry is continually expanding in its applications as a research tool.  Contact us to discuss what flow cytometry can do to help facilitate your research.

Services

Flow Cytometry has a diverse array of applications and the Research Institute at Nationwide Children’s Hospital Flow Cytometry Core seeks to help researchers with any application they wish to pursue. As science evolves, so does the scope of flow cytometry and the facility strives to stay abreast of the latest techniques.  The knowledgeable staff are here to assist you with experiment design, data acquisition, data analysis and any other questions you may have.

 

Applications

  •  Large particle sorting   
  •  CD34+  cell enumeration
  •  Chromosome sorting 
  •  Apoptosis
  •  Calcium flux ratio analysis 
  •  Gene expression
  •  Cell cycle analysis
  •  Membrane potential
  •  4 color fluorescent protein discrimination
  •  Oxidative metabolism (burst)
  •  Single and Multicolor immunophenotyping
  

 

Contacts

Name Role Phone Email Location
Dave Dunaway
Manager
 
614-355-2820
 
Dave.Dunaway@nationwidechildrenshospital.org
 
W290
 
Victoria Best
Manager
 
614-722-1393
 
Victoria.Best@nationwidechildrens.org
 
RB II