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Cell Line Core Lab

Overview of Services

The Cell Line Core processes human subject samples and provides the researcher with cell lines or DNA, appropriate for downstream applications in a variety of human and molecular genetic studies. Services include extraction of DNA from saliva or whole blood, creation of EBV transformed B-cell immortalized cell lines or fibroblast cell lines from skin biopsy samples, and DNA or cell line archiving. Additional tissue types may be processed on request. Samples can be provided directly to other related Cores (such as the Biomedical Genomic or High Throughput Genotyping Cores). The Core possesses a set of lymphoblastoid cell lines for use as normal controls in genotyping or gene expression studies. These Coriell samples include the North European CEPH subjects used in the HapMap project, which have a wealth of publicly available genotype and gene expression data. The Core can also assist investigators in the design of genetic studies on human subjects and biobanking.

Small volume samples for transformation: Blood samples can be difficult to obtain on children, and we frequently receive samples of only 1-2 ml. We have developed new protocols to handle samples of low volume and/or low WBC counts for lymphoblast transformation, with the expectation we can improve transformation in this difficult group from 90% up to 95%, to more closely match our success rate of optimal samples (currently at 98%).


The Biobank Human Control Resource:  The Cell Line Core currently has a control set consisting of a collection of CEPH (Centre d’ Etude Polymorphisme Humain) individuals of North European ancestry obtained by special agreement from the Coriel Institute.  This collection includes the parents of the trios used in the HapMap Project, plus some additional samples for a total of just over 100 individuals, all of European ancestry. We are in the process of establishing a Biobank Control Resource to expand this set in both numbers and ethnicity.  Our goal is to have up to 200 individuals of European and 200 of African American ancestry available. Other ethnic groups will be considered for collection if there is a need.

Leadership

Kim L. McBride, MD | Director

Location and hours of operation

Hours Location

Monday to Friday

8:30-5:00 

Research Bldg III, Room WB4350

Nationwide Children's Hospital

700 Children's Drive

Columbus, OH 43205

Links and Resources

Coriell Institute for Medical Research:  http://www.coriell.org/

ATCC: http://atcc.org/en.aspx

Contacts

Name Role Phone Email Location
Kim L. McBride, MD
Director
 
614 355 5759
 
kim.mcbride@nationwidechildrens.org
 
RB III Room WB4350
 
Reid Neinast
Research Assistant
 
617 355 5797
 
Reid.Neinast@nationwidechildrens.org
 
RB III Room WB4350
 

Services

Name Description Price
Project Request 

The Cell Line Core employs stringent Quality Control standards to ensure you receive high quality products.  When dealing with biological samples there is inherent variability. Sometimes successful derivation of a cell line does not occur.  We cannot assume responsibility for cell lines that refuse to grow due to low initial sample size, delays in sample delivery to our Core, inability to transform, or other reasons due to circumstances beyond our control.  The user thus assumes the possible risk of failed cell lines. In the rare case we fail to meet these standards, we will credit your account.  DNA/RNA products are created with the same stringent Quality Control standards, but in cases where the sample provided to the Cell Line Core is substandard, we cannot assume responsibility for poor outcomes.  

 

A list of services provided and prices for each service by the Cell Line Core are noted below.

 

By completing this form you agree to these conditions.  You also agree to the price list for the services you request.  No services will be provided until this form has been completed in full, and all required billing information has been provided. 

 
*
Cell line banking, per sample, per annum 

Maintain frozen cell line (-150 freezer duplicate; LN back-up single) for one year

 
*
Control Cell line 

Provide thawed cell line on Biobank Control Individual

 
*
Control DNA 96 well plate (large scale) 

Provide 50 micrograms DNA on Biobank Control Individual, 96 well plate

 
*
Control DNA 96 well plate (small scale) 

Provide 5 micrograms DNA on Biobank Control Individuals, 96 well plate

 
*
Control DNA Individual (large scale) 

Provide 50 micrograms DNA on Biobank Control Individual

 
*
Control DNA Individual (small scale) 

Provide 5 micrograms DNA on Biobank Control Individual

 
*
Cryopreservation peripheral WBCs    *
Culture fibroblasts  

Establish fibroblast cell line from skin biopsy sample; freeze down

 
*
Derive EBV transformed lymphoblastoid cell line 

Establish EBV-derived lymphoblastoid cell line from whole blood sample; freeze down

 
*
DNA Banking, per sample per annum 

Maintain banked DNA sample at -80

 
*
DNA Dilution 

Dilution of sample to investigator specifications

 
*
DNA extraction - blood 

Extract DNA from whole blood using PureGene kit

 
*
DNA extraction - Fibroblasts (cultured cells) 

Extract DNA from cultured fibroblast cells

 
*
DNA extraction - Lymphoblastoid cells 

Extract DNA from cultured lymphoblastoid cells

 
*
DNA extraction - Saliva 

Extract DNA from saliva (investigator provided collection kit)

 
*
DNA extraction - Tissue (skin, etc.) 

Extract DNA from tissue: skin biopsy, paraffin embedded formalin fixed tissue, etc.

 
*
DNA extraction - WBC (primary cells) 

Extract DNA from WBC separated from whole blood sample by Ficol method

 
*
Saliva collection kit with DNA extraction 

Extract DNA from saliva using Core provided Oragene collection kit

 
*
Total RNA extraction - Lymphoblastoid cells 

Extract RNA from cultured lymphoblastoid cells

 
*