PROCESSING AND BANKING CORE
PROCESSING AND BANKING
The Biospecimen Processing and Banking core provides a full range of biobanking services for researchers wishing to enhance their biospecimen collection and research efforts. Services include biospecimen accessioning, processing, nucleic acid extraction and quality control, banking, distribution, redaction of personally identifiable information, and digital pathology.
Researchers are encouraged to include the Biospecimen Processing and Banking core staff in the planning stages of their proposal as core staff can assist with creating a budget, reviewing feasibility, and lending their biobanking proficiency to ensure quality outcomes. The core can handle a wide variety of biospecimen types and maintain the flexibility needed to accommodate the unique needs of each project.
The Biopathology Center's molecular laboratory supports the nucleic acid extraction and quality control for the Biospecimen Processing and Banking Core and is accredited by the College of American Pathologists (CAP) Laboratory Accreditation Program and certified by CLIA for clinical testing. Other areas of the core are CAP-accreditated under their Biorepository Accrediation Program.
In addition, the Biopathology Center and the Biospecimen Processing and Banking Core is an active member of the International Society for Biological and Environmental Repositories (ISBER), and follows the highest standards to ensure the quality of specimens in the repository. Specimens are stored in liquid nitrogen, -80°C freezers, -20°C freezers, and 4°C refrigerators, which are monitored continuously to prevent the loss of these invaluable tissue samples.
DIGITAL PATHOLOGY SLIDE SCANNING
Digital Pathology offers histologic slide scanning services using Aperio scanners. The whole slide images can be generated at either 20X or 40X magnification.
RESEARCH FREEZER SERVICES
In addition to banking services offered by the Processing and Banking Core, researchers can store existing specimen collections by rack or entire freezer.
HISTOPATHOLOGY CORE
The Histopathology Core provides services in histology and routine transmission electron microscopy (TEM).
HISTOLOGY
The histology section of the core is accredited by the College of American Pathologists (CAP). It provides basic services such as tissue preparation and embedding, paraffin block or frozen tissue sectioning, and H&E staining. The core provides additional services such as direct and indirect immunohistochemical staining, selected histochemical stains, serial sectioning and RNase-free handling. Core personnel have extensive experience in tissue microarray construction. The lab is equipped with an MTA-1 manual Beecher TMA arrayer.
TEM
Routine TEM procedures such as fixation, embedding, and sectioning are available. The core utilizes a Hitachi H-7650 Transmission Electron Microscope (TEM) with two digital cameras. Magnification ranges from 0.7K up to 200K. The imaging software is AMtv542.
Dr. Diana Thomas, MD, PhD | Medical Director, Histopathology Core | Pathology Operations Director, Biopathology Center | Diana.Thomas@NationwideChildrens.org | 614-722-3705
Jay Bowen | Histopathology Core Director | Jay.Bowen@NationwideChildrens.org | 614-722-5302
Cindy McAllister | Histopathology Core Manager | Cynthia.McAllister@NationwideChildrens.org | 614-355-3465
Hours | Location |
7:30AM-5:00PM M-F |
Nationwide Children's Hospital 700 Children's Drive Columbus, Ohio 43205 Wexner- W230 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Histopathology Laboratory |
614-355-3474
|
Wexner- W284
|
||
Cindy McAllister |
Core Manager
|
614-355-3465
|
Cynthia.McAllister@NationwideChildrens.org
|
Wexner
|
Jay Bowen |
Core Director
|
614-722-5302
|
Jay.Bowen@NationwideChildrens.org
|
|
Dr. Diana Thomas, MD, PhD |
Medical Director of Core
|
614-722-3705
|
Diana.Thomas@NationwideChildrens.org
|
|
Megan Welling |
Processing and Banking Supervisor
|
614-355-3095
|
Megan.Welling@nationwidechildrens.org
|
Wexner
|
Andrew Mullen |
Digital Pathology Supervisor
|
614-355-
|
Andrew.Mullen@nationwidechildrens.org
|
|
Processing and Banking Laboratory |
614-355-2814
|
BPCCore@nationwidechildrens.org
|
Wexner- W230
|
Sample Preparation and Stains |
► Special Stains (1) | |||
Name | Description | Price | |
---|---|---|---|
Special Stain- Level 2- Luxol Fast Blue/ PAS |
Luxol fast Blue / PAS stain for slides. Price is per slide. |
OSU CCC
$35.00
slide
Academic/Non Profit $35.00 slide For Profit/All Other $57.00 slide NCHRI - Internal $25.00 slide |