Histopathology, Processing, and Banking Core

Overview of Services

PROCESSING AND BANKING CORE

PROCESSING AND BANKING

The Biospecimen Processing and Banking core provides a full range of biobanking services for researchers wishing to enhance their biospecimen collection and research efforts. Services include biospecimen accessioning, processing, nucleic acid extraction and quality control, banking, distribution, redaction of personally identifiable information, and digital pathology. 

Researchers are encouraged to include the Biospecimen Processing and Banking core staff in the planning stages of their proposal as core staff can assist with creating a budget, reviewing feasibility, and lending their biobanking proficiency to ensure quality outcomes. The core can handle a wide variety of biospecimen types and maintain the flexibility needed to accommodate the unique needs of each project.

The Biopathology Center's molecular laboratory supports the nucleic acid extraction and quality control for the Biospecimen Processing and Banking Core and is accredited by the College of American Pathologists (CAP) Laboratory Accreditation Program and certified by CLIA for clinical testing. Other areas of the core are CAP-accreditated under their Biorepository Accrediation Program.

In addition, the Biopathology Center and the Biospecimen Processing and Banking Core is an active member of the International Society for Biological and Environmental Repositories (ISBER), and follows the highest standards to ensure the quality of specimens in the repository. Specimens are stored in liquid nitrogen, -80°C freezers, -20°C freezers, and 4°C refrigerators, which are monitored continuously to prevent the loss of these invaluable tissue samples.

DIGITAL PATHOLOGY SLIDE SCANNING

Digital Pathology offers histologic slide scanning services using Aperio scanners. The whole slide images can be generated at either 20X or 40X magnification.

RESEARCH FREEZER SERVICES

In addition to banking services offered by the Processing and Banking Core, researchers can store existing specimen collections by rack or entire freezer.

 

HISTOPATHOLOGY CORE

The Histopathology Core provides services in histology and routine transmission electron microscopy (TEM).

HISTOLOGY

The histology section of the core is accredited by the College of American Pathologists (CAP). It provides basic services such as tissue preparation and embedding, paraffin block or frozen tissue sectioning, and H&E staining. The core provides additional services such as direct and indirect immunohistochemical staining, selected histochemical stains, serial sectioning and RNase-free handling. Core personnel have extensive experience in tissue microarray construction. The lab is equipped with an MTA-1 manual Beecher TMA arrayer.

TEM

Routine TEM procedures such as fixation, embedding, and sectioning are available. The core utilizes a Hitachi H-7650 Transmission Electron Microscope (TEM) with two digital cameras. Magnification ranges from 0.7K up to 200K. The imaging software is AMtv542.

  

Leadership

Dr. Diana Thomas, MD, PhD | Medical Director, Histopathology Core | Pathology Operations Director, Biopathology Center | Diana.Thomas@NationwideChildrens.org | 614-722-3705

Jay Bowen | Histopathology Core Director | Jay.Bowen@NationwideChildrens.org | 614-722-5302

Cindy McAllister | Histopathology Core Manager | Cynthia.McAllister@NationwideChildrens.org | 614-355-3465

 

Location and hours of operation

Hours Location
7:30AM-5:00PM  M-F  

Nationwide Children's Hospital

700 Children's Drive

Columbus, Ohio 43205

Wexner- W230

Links and Resources

    1. http://www.nationwidechildrens.org/morphology-core

 

Contacts

Name Role Phone Email Location
Histopathology Laboratory

 
614-355-3474
 

 
Wexner- W284
 
Cindy McAllister
Core Manager
 
614-355-3465
 
Cynthia.McAllister@NationwideChildrens.org
 
Wexner
 
Jay Bowen
Core Director
 
614-722-5302
 
Jay.Bowen@NationwideChildrens.org
 

 
Dr. Diana Thomas, MD, PhD
Medical Director of Core
 
614-722-3705
 
Diana.Thomas@NationwideChildrens.org
 

 
Megan Welling
Processing and Banking Supervisor
 
614-355-3095
 
Megan.Welling@nationwidechildrens.org
 
Wexner
 
Andrew Mullen
Digital Pathology Supervisor
 
614-355-
 
Andrew.Mullen@nationwidechildrens.org
 

 
Processing and Banking Laboratory

 
614-355-2814
 
BPCCore@nationwidechildrens.org
 
Wexner- W230
 

Sample Preparation and Stains


Search available services: View: by category alphabetically
Special Stains (1)